Job Request

1. Target DNA sequence at a genomic locus 

  • 2. Guide RNA sequence 

  • 3. Search range from putative cleavage site 

     nt (–)

     nt (+)

    4. Maximum size for microhomology 

  • 5. E-mail address (Optional) 

    How to use:

    If you click the question mark " ", the instructions will be diplayed here.


    This web-based program finds two local microhomologies (3-6 bp in length) to predict potential deletion patterns induced by engineered nucleases CRISPR.

    1. Enter your target region of genomic DNA (50-500 bp in length).

    2. Enter your CRISPR/Cas9 targeting sequences (10-30 bp in length) that exist in the above target region.

    3. Press "Compute" to get results.

    Sample data: