Welcome to In silico Genome Editing and Analysis Tools


The In Silico Genome Editing and Analysis Tools (iGEATs) provide users to search for local-microhomologies to predict deletion sizes induced by engineered nucleases.

Engineered nucleases, such as TALENs or CRISPR/Cas9 systems, generate small deletions at the desired site. The sizes of the deletion is basically random, but certain sizes of the deletion which mediated with local microhomology sequences are one of the most major deletion patterns.

Predict deletion patterns mediated by microhomology
Search for microhomologies flanked by your nuclease of interest to predict deletion patterns here.

Find unique target region in the genome
Display the "uniqueness" of a particular genomic region of interest by using the short sequences that only matched to the genome uniquely. Higher peaks mean higher uniqueness, and such region is a good target for designing custom engineering nucleases or PCR primers here.

Latest Release:

iGEATs Ver1.0.5 was released Sep 17, 2015

Mouse Assembly types available on the service Find Uniq Sequence.

- iGEATs Ver1.0.4 was released Aug 21, 2014

- iGEATs Ver1.0.3 was released Aug 19, 2014

- iGEATs Ver1.0.2 was released Aug 16, 2014

- iGEATs Ver1.0.1 was released Aug 12, 2014


In silico Genome Editing Tools Ver 1.0 was released

- Opened Aug 1, 2014

Citing iGEATs:

If you find iGEATs useful, please consider citing
the reference that describes this work:

Hongmei Lisa Li, Naoko Fujimoto, Noriko Sasakawa, Saya Shirai, Tokiko Ohkame, Tetsushi Sakuma, Michihiro Tanaka, Naoki Amano, Akira Watanabe, Hidetoshi Sakurai, Takashi Yamamoto, Shinya Yamanaka, Akitsu Hotta. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports 2015 4(1):143-154