Introduction
Engineered nucleases, such as TALENs or CRISPR/Cas9 systems, generate small deletions at the desired site. The sizes of the deletion is basically random, but certain sizes of the deletion which mediated with local microhomology sequences are one of the most major deletion patterns.

Predict deletion patterns mediated by microhomology
Search for microhomologies flanked by your nuclease of interest to predict deletion patterns here.

Find unique target region in the genome
Display the "uniqueness" of a particular genomic region of interest by using the short sequences that only matched to the genome uniquely. Higher peaks mean higher uniqueness, and such region is a good target for designing custom engineering nucleases or PCR primers here.